The updated manual is not a revision of features but a crucial document for a legacy instrument. While the core functionality remains unchanged, the key "updates" that a user of a second-hand 5402 would look for are:
Follow these step-by-step procedures to ensure a safe, efficient, and reproducible separation workflow. Pre-Run Setup
The standard 24-place fixed-angle rotor is autoclavable at 121°C for 20 minutes if biological decontamination is necessary. Material Compatibility Warnings
Press down firmly on the lid. Inspect the latch mechanisms for mechanical blockages. Condenser coils are blocked or ambient room air is too hot. eppendorf 5402 manual updated
If power fails and your samples are trapped inside, locate the manual emergency release slot (usually a small pinhole on the side or bottom of the unit). Insert a standard manual release tool or screwdriver to trip the latch. Never attempt this while the rotor is spinning. 4. Routine Maintenance and Care
Press STOP immediately. Wait for standstill. Clean spilled liquids and re-balance tubes using a scale. Condenser coils blocked; lid seal leaking; low refrigerant.
Earlier 5402 models utilize carbon brushes. The updated manual is not a revision of
Pre-cooling capability with a rapid "Fast Cool" function Interface Panel and Controls
The centrifuge will automatically stop and indicate the end of the cycle. The lid lock will release automatically when the rotor comes to a complete stop. 3. Maintenance and Care
Comprehensive Guide to the Eppendorf 5402 Refrigerated Microcentrifuge Go to product viewer dialog for this item. : Updated Manual & Best Practices The Eppendorf 5402 refrigerated centrifuge Go to product viewer dialog for this item. Material Compatibility Warnings Press down firmly on the
If the unit has been moved from a cold environment to a warmer one, allow it to sit for at least 4 hours to avoid moisture condensation on internal electronics.
Ensure opposite tubes are equal in weight, not just fluid volume. Density differences matter.